WVSU Research Journal, 05(02), December 2016

Influence of fermented organic concoction as nutrient supplements in the performance of brooding native chicken

Thu, 01 Dec 2016 00:00:00 GMT

Influence of fermented organic concoction as nutrient supplements in the performance of brooding native chicken Huervana, Jonnie F. The study determined the alternatives of producing brooder chicks without antibiotics by utilizing organic concoctions as nutrient supplements and as preventive measures against diseases. The experimental study assigned in Randomized Complete Block Design with 100 chicks as the subject of the experiment. Each treatment has 20 birds with five birds per replicate. The five treatments were: Treatment A- water only, Treatment B- antibiotics with vitamins, Treatment C- fermented banana fruit juice, Treatment D- fermented pineapple fruit juice, and Treatment E- fermented banana plant juice. Fermented concoctions were added and mixed to the daily water requirement of birds. The result of the study revealed significant differences among the treatments regarding feed consumption. Least Significant Differences (LSD) indicates that birds given with fermented pineapple juice through the drinking water significantly consumed lesser feeds compared to other treatments. Other parameters such as survival rate, feed conversion ratio (FCR), final live weight, the gain in weight, and water consumption were not significant. The results imply that drinking water of birds using organically fermented concoction as nutrient supplements, or pure water or with vitamins and antibiotics were all comparable regarding gain in weight, final live weight, feed conversion ratio, and survival rate of chicks during the brooding period of 21 days.

Phytochemical and antimicrobial analysis of “lupo” (Alternanthera sessilis L. R. Br.)

Thu, 01 Dec 2016 00:00:00 GMT

Phytochemical and antimicrobial analysis of “lupo” (Alternanthera sessilis L. R. Br.) Monroy, Anita Estela M.; Limsiaco, Cynthia L. In this study, the presence of selected phytochemicals from the aqueous, acetone, and ethanolic extracts was screened and the antimicrobial and antifungal potentials of the expressed juice of “Lupo” (Alternanthera sessilis) may be possible nutritional and medicinal values. The extracts were subjected to the tests for Alkaloids,Steroids, Anthraquinones, Flavonoids, and Tannins, using standard procedures in Guevara’s, A Guidebook to Plant Screening: Phytochemical and Biological,2005. The Kirby-Bauer technique of minimum antibacterial concentration and minimum inhibitory concentration (MIC) were used in the assay. Results show that alkaloids, flavonoids, steroids, and anthraquinones, are present in all extracts of Alternanthera sessilis, while tannins is only in the aqueous extract. Alternanthera sessilis when extracted using water contains all the five phytochemicals tested, and is beneficial when mixed in food products, because it can supply alkaloids and steroids that exhibit dramatic physiological effects on the heart muscles. It can also supply natural cathartics or purgatives due to the presence of anthraquinones. Flavonoids present have antiviral, anti-fungal, anti-inflammatory and cytotoxic activities. Tannins present in aqueous extract have potential value as cytotoxic and/ or antineoplastic agents, and as astringents. Results of antimicrobial and antifungal analyses show that Alternanthera sessilis(Lupo) expressed juice possess an antibacterial activity exhibiting an MIC of < 100µg/mL against Escherichia coli referenced strain used. There is low MIC of 252.5µg/mL against Staphylococcus aureus and Candida albicans.

Isolation, screening, and identification of amylase-producing fungi from selected rice mills for starch degradation

Thu, 01 Dec 2016 00:00:00 GMT

Isolation, screening, and identification of amylase-producing fungi from selected rice mills for starch degradation Tantiado, Rey G. This study was conducted to isolate, screen, characterize, and identify amylase-producing fungi from selected rice mills in Iloilo for the degradation of starch. Amylases are classes of enzymes which have useful applications in various commercial industries with the prospering industry in Iloilo City. There were three rice mill sampling sites considered in this study. Serial dilution and spread plate method were used to isolate and characterize soil fungi from the three rice mills. A total of seven amylolytic soil fungi were isolated and identified as degraders of starch. Iodine’s test after seventy-two hours of incubation was used to screen the amylolytic activity among the isolates. The range of starch degradation was described from slightly effective to very effective results. Slide culture technique was then, used for characterization and identification of active isolates, and were identified under the genera of Aspergillus, Penecillum and Alternaria species. Based on the result of the study, isolate 7, Penicillium spp. had the highest zone of amylolytic activity while isolate 3, Aspergillus spp. had the lowest zone of amylolytic activity. There was also a significant difference on the zone of amylolytic activity that existed among the fungi isolates after 72 hours of incubation using Iodine test.