Marine sediment-derived Streptomyces strain produces angucycline antibiotics against multidrug-resistant Staphylococcus aureus harboring SCCmec type 1 gene
Date
2020-09-23Author
Taxonomic term
Streptomyces marinus![Global Biodiversity Information Facility GBIF](/themes/Mirage2/images/gbif-16x16.png)
Staphylococcus aureus
![Global Biodiversity Information Facility GBIF](/themes/Mirage2/images/gbif-16x16.png)
Streptomyces
![Global Biodiversity Information Facility GBIF](/themes/Mirage2/images/gbif-16x16.png)
Staphylococcus
![Global Biodiversity Information Facility GBIF](/themes/Mirage2/images/gbif-16x16.png)
Streptomyces griseorubens
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Abstract
The Philippine archipelago is geographically positioned in the tropics with rich areas of marine biodiversity. Its marine sediments harbor actinomycetes that exhibit antibacterial activity. Screening of actinomycetes isolated from marine sediments collected near the coast of Islas de Gigantes, Iloilo showed one isolate that exhibited high activity against the multidrug-resistant Staphylococcus aureus (MRSA) strain carrying the Staphylococcal Cassette Chromosome mec (SCCmec) type 1 gene, a biomarker for drug resistance. The isolate was identified as Streptomyces sp. strain DSD011 based on its 16s rRNA and protein-coding genes (atpD, recA, rpoB, and trpB) sequences, and was found to be a new species of salt-tolerant marine Streptomyces. Further, the strain harbors both non-ribosomal peptide synthetase (NRPS) and type II polyketide synthase (PKS) in its genome. The targeted chromatographic isolation and chemical investigations by Liquid Chromatography Mass Spectrometry-Time of Flight (LCMS-TOF), tandem mass spectrometry (MS/MS), and Global Natural Product Social molecular networking (GNPS) of the antibiotics produced by the strain afforded the two polycyclic aromatic polyketide angucycline glycosides, fridamycin A (1) and fridamycin D (2), which are products of type II PKS biosynthesis. Compounds 1 and 2 displayed antibacterial activity against MRSA with minimum inhibitory concentration (MIC) of 500 μg/mL and 62.5 μg/mL, respectively. These results suggest that the underexplored marine sediments near the coast of Islas de Gigantes, Iloilo offer access to undiscovered Streptomyces species that are invaluable sources of antibiotic leads.
Recommended Citation
Sabido, E. M., Tenebro, C. M., Suarez, A. F. L., Ong, S. D. C., Trono, D. J. V. L., Amago, D. S., ... & Dalisay, D. S. (2020). Marine sediment-derived Streptomyces strain produces angucycline antibiotics against multidrug-resistant Staphylococcus aureus harboring SCCmec type 1 gene.Type
ArticleISSN
20771312Keywords
Marine Sediments Antibiotic angucycline glycosides Multidrug-resistant Staphylococcus aureus SCCmec gene Type II polyketide synthase MRSA SCCmec Staphylococcal Cassette Chromosome mec Liquid Chromatography Mass Spectrometry-Time of Flight LCMS-TOF tandem mass spectrometry MS/MS Global Natural Product Social molecular networking GNPS Islas de Gigantes antibiotic leads Chromatographic isolation Salinispora tropica Salinosporamide A Marine actinomycetes Antibiotic anthracycline shunt metabolites bisanhydroaklavinone 1-hydroxybisanhydroaklavinone Philippine marine sediment Actinomycete Strain DSD011