Association of circulating serum miR-34a and miR-122 with dyslipidemia among patients with non-alcoholic fatty liver disease
dc.contributor.author | Salvoza, Noel C. | |
dc.contributor.author | Klinzing, David C. | |
dc.contributor.author | Gopez-Cervantes, Juliet | |
dc.contributor.author | Baclig, Michael O. | |
dc.date.accessioned | 2021-12-29T04:13:57Z | |
dc.date.available | 2021-12-29T04:13:57Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Salvoza, N. C., Klinzing, D. C., Gopez-Cervantes, J., & Baclig, M. O. (2016). Association of circulating serum miR-34a and miR-122 with dyslipidemia among patients with non-alcoholic fatty liver disease. PLoS ONE, 11(4), e0153497. | en |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | http://repository.wvsu.edu.ph/handle/123456789/68 | |
dc.description.abstract | Non-alcoholic fatty liver disease (NAFLD) covers a spectrum of diseases from simple steatosis to non-alcoholic steatohepatitis, with approximately 20% risk of progressing to fibrosis and cirrhosis. The aim of this study was to compare the relative expression levels of circulating miR-21, miR-34a, miR-122, miR-125b and miR-375 between healthy controls and NAFLD patients, and to assess the feasibility of microRNAs as potential biomarkers for NAFLD. A cross-sectional study was conducted to evaluate circulating serum miRNAs as potential diagnostic markers for NAFLD. Twenty-eight clinically diagnosed and histologically-confirmed NAFLD patients, as well as 36 healthy controls were enrolled in this study. The relative expression of serum microRNAs were calculated using the comparative cycle threshold with spiked-in C. elegans miR-39 as exogenous internal control. Serum levels of miR-34a and miR-122 were significantly higher in NAFLD patients than in healthy controls (P = <0.0001). Positive correlations were observed between serum miR-34a with very low density lipoprotein cholesterol (VLDL-C) and triglyceride levels. However, the expression levels of miR-34a and miR-122 did not correlate with the histological features of NAFLD. Interestingly, receiver operating characteristic (ROC) curve analysis revealed that miR-34a and miR-122 are potential markers for discriminating NAFLD patients from healthy controls with an area under the curve (AUC) values of 0.781 and 0.858, respectively. Serum levels of miR-34a and miR-122 were found to be significantly higher among NAFLD patients, and were positively correlated with VLDL-C and triglyceride levels. Thus, circulating miR-34a and miR-122 can be used as potential biomarkers for discriminating NAFLD patients from healthy controls. Larger cohorts are required to validate the utility of miR-34a and miR-122 in monitoring liver injury. | en |
dc.description.sponsorship | This work was supported by a research grant to the St. Luke’s Liver Diseases Study Group (Project no. 12-002a) through the Research and Biotechnology, St. Luke’s Medical Center-Quezon City. | en |
dc.language.iso | en | en |
dc.publisher | Public Library of Science | en |
dc.relation.uri | en | |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject.lcsh | Fatty liver | en |
dc.subject.lcsh | Liver--Diseases | en |
dc.subject.lcsh | Lipids--Metabolism | en |
dc.subject.mesh | Fatty Liver | en |
dc.subject.mesh | Non-alcoholic Fatty Liver Disease | en |
dc.subject.mesh | Fibrosis | en |
dc.subject.mesh | Histology | en |
dc.subject.mesh | Lipoproteins | en |
dc.subject.mesh | MicroRNAs | en |
dc.subject.mesh | Lipid Metabolism | en |
dc.title | Association of circulating serum miR-34a and miR-122 with dyslipidemia among patients with non-alcoholic fatty liver disease | en |
dc.type | Article | en |
dc.citation.journaltitle | PLoS ONE | en |
dc.citation.volume | 11 | en |
dc.citation.issue | 4 | en |
dc.citation.firstpage | e0153497 | en |
dc.identifier.doi | 10.1371/journal.pone.0153497 |
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